Titlebook: Imaging Flow Cytometry; Methods and Protocol Natasha S. Barteneva,Ivan A. Vorobjev Book 2016 Springer Science+Business Media New York 2016

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Applications of Imaging Flow Cytometry for Microalgaelity to image intracellular features adds a unique aspect to analyses, and can enable correlation between molecular phenomena resulting in alterations in cellular phenotype. Unicellular microalgae are amenable to high-throughput analysis to capture the diversity of cell types in natural samples, or

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Fluorescent In Situ Hybridization in Suspension by Imaging Flow Cytometry. One of the new applications is fluorescence in situ hybridization in suspension (FISH-IS). Conventional FISH is a slide-based approach in which the spotlike imagery resulting from hybridization with fluorescently tagged probes is evaluated by fluorescence microscopy. The FISH-IS approach evaluated

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Analysis of Nucleocytoplasmic Protein Shuttling by Imaging Flow Cytometrysses requires imaging information on the protein localization in a given cell and a large number of measurements to obtain sufficient statistics on the protein localization in the whole population. The protocol describes method for quantitative imaging flow cytometry analysis of intracellular distri

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Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellulack the ability to simultaneously detect intracellular mRNA and protein. This procedure takes advantage of new methodologies and instrumentation to simultaneously measure intracellular TNF-α mRNA and protein in CD14. monocytes after 1, 3, and 6 h of LPS stimulation. By assessing multiple timepoints

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Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry that lack image-based analysis capabilities fail to fully evaluate granulocyte function. In the present method, we combine simultaneous detection of phagocytosis and oxidative burst in granulocytes to identify unique subsets of activated granulocytes. This analysis method provides novel information

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