Titlebook: Icons of the Alphabet; Letter Names, Phonet Reese M. Heitner Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive lic

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Reese M. Heitnerchemical variability that could be potentially advantageous for functional diversification of proteins, especially in a user-defined environment. Recent examples of such artificial (i.e., tailor-made) proteins include functional design of novel classes of protein pH sensors, novel classes of green f

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the rapid translation system (RTS), an .-based cell-free system. The proteins selected for this feasibility study represent four classes of membrane proteins that are found in organelles, involved in endo-and exocytosis (Fig. 16.1A) (reviewed in High and Laird 1997), i.e., a type-I membrane protein

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cal, .Cell‐Free Gene Expression: Methods and Protocols. serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications..978-1-0716-2000-7978-1-0716-1998-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Reese M. Heitner gradient. This method also includes a protocol to pause and restart translation of membrane proteins at specified positions during their co-translational folding. This stop–start strategy provides an avenue to investigate whether the proteins fold in sequence order, or if the correct fold of N-term

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n milligram amounts in the RTS 500. HY. The non-native proteins were used for immunization and as protein standards for quantitation of the respective proteins in biological material by semi-quantitative Western-blotting. Along the way we also learned some interesting details about the rapid transla

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