Titlebook: ICT Education; 45th Annual Conferen Stefan Gruner Conference proceedings 2016 Springer International Publishing AG 2016 classrooms.collabor

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Bob Travicaprotocol for the implementation of CRISPR-mediated wheat gene editing and trait improvement. We begin with gRNA designing using online tools, followed by assembling CRISPR constructs or ribonucleoprotein (RNP) complex and in vitro and in vivo gRNA validation using wheat mesophyll protoplasts. Furthe

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Melisa Koorsse,Marinda Taljaard,André P. Calitze that is further trimmed to generate the mature crRNAs. Type II systems have evolved a unique crRNA biogenesis pathway, in which a .-acting small RNA (encoded by the CRISPR-Cas locus) base pairs with each repeat sequence of the pre-crRNA to form a double-stranded RNA template that is cleaved by the

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Ken Hallande sequence comparison aided by analysis of the available crystal structures led to the delineation of approximately 30?protein families that can be further classified into several superfamilies. By far the most common domain in Cas proteins is the RNA Recognition Motif (RRM). The RRM domains show re

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Melisa Koorsse,André P. Calitz,Jaco Zietsmanake them amenable to genetic engineering and useful for genome editing. These features, in combination with recent breakthroughs in unravelling the molecular underpinnings of the CRISPR mechanism of action have paved the way for several applications in a diversity of industrial and biotechnological

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Fritz Solms,Vreda Pietersestered .egularly .nterspersed .hort .alindromic .epeats and . for CRISPR associated proteins), is becoming a tool of choice, due to its simplicity, versatility, scalability and lower cost when compared to other biotechnological tools. Improved versions of CRISPR/Cas-GE, such as base editing are comi

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On the Morality of Teaching Students IT Crime Skillsledge to harm and knowledge to help overlap in many professional contexts. The lecture argues questions on the morally of imparting potentially malicious knowledge should differentiate between imparting it to those entering a profession and imparting it to the masses. While this does not prevent the

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A Comparison of E-Assessment Assignment Submission Processes in Introductory Computing Coursesed suggestions for improving the on-line submission system. The results could assist educators and instructors utilising e-assessment systems in improving the submission and marking processes, in any course where files are required for submission.

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