Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Synthetic and System Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2016 Springer-Ve

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Protocol for the Standardisation of Transcriptional Measurements,One of the most important areas to standardise is the measurement of part, device and system activity in order to improve designs and aid sharing of data. While methods for standardising transcriptional measurements have been designed, they have suffered from poor uptake, and as more parts and syste

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Uracil Excision for Assembly of Complex Pathways,nd synthetic biology. Simplicity and robustness are attractive features met by the uracil excision DNA assembly method, which is one of the most inexpensive technologies available. Here, we describe four different protocols for uracil excision-based DNA editing: one for simple manipulations such as

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Phenome-ing Microbes,rganism’s isolate arise through simple sequence variations like single nucleotide polymorphisms (SNP) or small insertions/deletions (InDel). For some phenotypes, however, the underlying mechanism cannot be explained by simple genomic differences; rather, most of them are the result of more complex s

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A Practical Protocol for Integration of Transcriptomics Data into Genome-Scale Metabolic Reconstruc this huge volume of data is challenging from a purely mathematical and statistical point of view, and integrative approaches are becoming a necessity. Genome-scale metabolic models offer an unprecedented chance to integrate and contextualise, in the correct biological context, this large amount of

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