| 書(shū)目名稱 | Hydrocarbon and Lipid Microbiology Protocols | | 副標(biāo)題 | Microbial Quantitati | | 編輯 | Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale | | 視頻video | http://file.papertrans.cn/431/430315/430315.mp4 | | 概述 | Offers readily reproducible, step-by-step laboratory methods.Provides helpful tips and tricks to complement the protocols.Includes troubleshooting advice.Features practical laboratory guidelines to pr | | 叢書(shū)名稱 | Springer Protocols Handbooks | | 圖書(shū)封面 |  | | 描述 | .This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers...Hydrocarbon and Lipid Microbiology Protocols.There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganism | | 出版日期 | Book 2017 | | 關(guān)鍵詞 | Microbial communities; Oily sediments; Hydrocarbon-contaminated environments; Hydrocarbon-degrading bac | | 版次 | 1 | | doi | https://doi.org/10.1007/978-3-662-52778-8 | | isbn_softcover | 978-3-662-57081-4 | | isbn_ebook | 978-3-662-52778-8Series ISSN 1949-2448 Series E-ISSN 1949-2456 | | issn_series | 1949-2448 | | copyright | Springer-Verlag Berlin Heidelberg 2017 |
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