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Titlebook: Human Retrovirus Protocols; Virology and Molecul Tuofu Zhu Book 2005 Humana Press 2005 DNA.Microarray.PCR.RNA.Termination.gene expression.g

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發(fā)表于 2025-3-21 20:03:42 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱Human Retrovirus Protocols
副標(biāo)題Virology and Molecul
編輯Tuofu Zhu
視頻videohttp://file.papertrans.cn/430/429428/429428.mp4
概述Includes supplementary material:
叢書(shū)名稱Methods in Molecular Biology
圖書(shū)封面Titlebook: Human Retrovirus Protocols; Virology and Molecul Tuofu Zhu Book 2005 Humana Press 2005 DNA.Microarray.PCR.RNA.Termination.gene expression.g
描述A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
出版日期Book 2005
關(guān)鍵詞DNA; Microarray; PCR; RNA; Termination; gene expression; genes; molecular biology; reproducible techniques; v
版次1
doihttps://doi.org/10.1385/1592599079
isbn_softcover978-1-61737-599-6
isbn_ebook978-1-59259-907-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2005
The information of publication is updating

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Quantification of HFV-Integrated DNA in Human Cells by ,-LTR Real-Time PCRf the amplification curve of the sample against a standard scale constituted of viral DNA from chronically infected cells. Sensitivity of this technique allows us to detect as few as 25 copies of HFV-integrated DNA in 50,000 cells.
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Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretionsual HIV transmission. Additional investigations have found a significant correlation between the viral load in blood plasma and in the genital tract. This chapter describes methods of collection, processing, and testing in blood, plasma, and male and female genital secretions for quantifying HIV RNA.
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Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Car-cells from HIV-1-infected individuals, activating the cells to induce virus production, and detecting and quantitating cells capable of releasing infectious virus. The development of an enhanced viral culture assay to quantitate the number of latently infected cells carrying replication competent virus is described here.
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Isolation and Quantification of HIV From Lymph Nodes cocultivated with phytohemagglutinin-stimulated normal donor PBMC for 21 d. The supernatant from each well is assayed for HIV p24 antigen production by the standard HIV p24 enzyme-linked immunosorbent assay (ELISA). HIV-1 infectious titers are expressed as tissue culture infective dose (TCID./10. cells) according to Reed and Muench.
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1064-3745 iology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolat
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