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Titlebook: Human Respiratory Syncytial Virus; Methods and Protocol Ralph A. Tripp,Patricia A. Jorquera Book 2016 Springer Science+Business Media New Y

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書目名稱Human Respiratory Syncytial Virus
副標(biāo)題Methods and Protocol
編輯Ralph A. Tripp,Patricia A. Jorquera
視頻videohttp://file.papertrans.cn/430/429424/429424.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Human Respiratory Syncytial Virus; Methods and Protocol Ralph A. Tripp,Patricia A. Jorquera Book 2016 Springer Science+Business Media New Y
描述.Thisvolume summarizes current techniques; ranging from protocols for virus growth,isolation, quantification and generation of recombinant RSV virus to proceduresfor the efficient characterization of the host immune response to RSVinfection. These techniques are used in numerous laboratories around the worldand are the building blocks that support the majority of RSV virus research. Written in the highly successful .Methods in MolecularBiology .series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls...Authoritative and cutting-edge,. Humana Respiratory Syncytial Virus:Methods and Protocols .aims to ensure successful resultsin the further study of this vital field..
出版日期Book 2016
關(guān)鍵詞virus growth; isolation; RSV virus; immune response; RSV infection; lower respiratory tract infections; in
版次1
doihttps://doi.org/10.1007/978-1-4939-3687-8
isbn_softcover978-1-4939-8117-5
isbn_ebook978-1-4939-3687-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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RSV Growth and Quantification by Microtitration and qRT-PCR Assays,virulence of the infection. Here, we describe a simple method, based on limiting dilution, for the removal of defective interfering particles from RSV. This method results in a high-titer viral preparation from both HEp-2 and Vero cell lines. We evaluated two concentrations of sucrose to stabilize t
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