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Titlebook: Human Na?ve Pluripotent Stem Cells; Peter Rugg-Gunn Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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發(fā)表于 2025-3-21 19:12:44 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)Human Na?ve Pluripotent Stem Cells
編輯Peter Rugg-Gunn
視頻videohttp://file.papertrans.cn/430/429335/429335.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Human Na?ve Pluripotent Stem Cells;  Peter Rugg-Gunn Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to
描述.This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use na?ve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate na?ve hPSC lines; methods to differentiate na?ve hPSCs into specialized cell types; and techniques to characterize na?ve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Cutting-edge and thorough, .Human Na?ve Pluripotent Stem Cells: Methods and Protocols. is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field..
出版日期Book 2022
關(guān)鍵詞reprogramming; na?ve pluripotency; preimplantation embryos; transcriptional datasets; Crispr-Cas9
版次1
doihttps://doi.org/10.1007/978-1-0716-1908-7
isbn_softcover978-1-0716-1910-0
isbn_ebook978-1-0716-1908-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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,Generating Trophoblast Stem Cells from Human Na?ve Pluripotent Stem Cells,s indispensable for a healthy pregnancy. Epithelial cells in the placenta, which are termed trophoblasts, originate from the trophectoderm (TE) compartment of the blastocyst. The human trophoblast lineage consists of several distinct cell types, including the self-renewing and bipotent cytotrophobla
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,Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Na?ve Pluripotent Suring blastocyst development, and the second being gastrulation-stage definitive endoderm (DE). The PrE gives rise to the primary and secondary yolk sac, and has supportive functions during pregnancy for nutrient provision, with descendants of this extra-embryonic lineage also playing a role in embr
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,Capacitation of Human Na?ve Pluripotent Stem Cells,ibes a simple experimental system for the efficient and consistent transition of human pluripotent stem cells (hPSCs) from the na?ve to the primed state, which is a process called capacitation. Na?ve hPSCs after capacitation can be differentiated further to somatic lineages, thus reproducing the ord
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