Titlebook: Host-Pathogen Interactions; Methods and Protocol Carlos Medina,Francisco Javier López-Baena Book 2018 Springer Science+Business Media, LLC

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Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/428421.jpg

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,Genetic Association Studies in Host–Pathogen Interaction Analysis,thways involved in the different stages of infection traditionally require complex molecular biology tools and often the use of costly animal models. In this chapter we illustrate a complementary approach to address host–pathogen interactions, taking advantage of the natural interindividual genetic

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Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogge of RNA reverse transcription to generate complementary DNA (cDNA), this is a powerful approach to determine and analyze gene transcriptional expression. Real-time quantitative reverse transcription PCR has become the gold standard method in studying genes expression and virulence regulation under

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Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection. Currently available technology allows for the exploitation of a bioluminescent reporter system to study bacterial gene regulation during rodent infection, in real time, over a large dynamic range. Here we show how this imaging system can be used to study virulence gene regulation during . infectio

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Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogensnd other pathogens under various conditions. This method includes a preliminary stage of fusing the target promoter to a promoter-less . gene encoding for the enzyme .-galactosidase. Supplementation of the synthetic ONPG substrate results in the accumulation of a chromogenic product proportionally t

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Western Blotting Against Tagged Virulence Determinants to Study Bacterial PathogenicityThis method is also useful to study regulation of virulence determinants by analyzing changes in protein expression between different genetic backgrounds or under varying environmental conditions. To avoid the need to raise specific antibodies for each studied protein, commercial antibody against co

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Molecular Methods to Analyze the Effect of Proteins Expressed by , During Its Intracellular Stagefection process known as effectors that translocate through its own secretion systems to the eukaryotic cytosol exerting diverse effects on the cell. Additionally, . can be engineered to include a protein expression system that, upon the addition of an inducer molecule, can produce heterologous prot