Titlebook: Hormone Assays in Biological Fluids; Michael J. Wheeler,J. S. Morley Hutchinson Book 2006 Humana Press 2006 Chromatography.Mass Spectromet

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Measurement of Aldosterone in Blood,d iodinated aldosterone. Details are given for optimizing conditions for the second antibody, raised to rabbit immunoglobulins, and carrier protein used in the separation of the antibody bound fraction from the free aldosterone at the end of equilibration. Details are provided in additional notes, for steps in the assay that could be problematic.

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Book 2006riety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, a

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Book 2006l needs.Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

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Urinary Steroid Profiling,e large quantities of sulphates, so conjugate separation by liquid gel chromatography is used prior to hyrolysis. Normal ranges for adults and children are given, together with advice on chromatogram evaluation and a summary of the profile findings in steroid disorders.

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High-Performance Liquid Chromatography for Hormone Assay,tion limit is only nanogram amounts of hormones per milliliter of blood serum. The applications of the technique to specific hormones from recent and older literature will be used throughout this chapter to illustrate aspects of the technology.