書目名稱 | Histone Methyltransferases | 副標(biāo)題 | Methods and Protocol | 編輯 | Rapha?l Margueron,Daniel Holoch | 視頻video | http://file.papertrans.cn/428/427290/427290.mp4 | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 叢書名稱 | Methods in Molecular Biology | 圖書封面 |  | 描述 | .This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through?a?comprehensive set of approaches that detail?phylogenetic diversity, histone demethylase activities?.in vitro,.?generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy,?assessing?genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and?development of methyltransferase inhibitors.?Written in the format of the highly successful?.Methods in Molecular Biology?.series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols...?..Authoritative and cutting-edge,?.Histone Methyltransferases: Methods and Protocols?.aims?to be a?useful and practical guide to new researchers?and experts looking to expand their knowledge.. | 出版日期 | Book 2022 | 關(guān)鍵詞 | chromatin compaction; automethylation; substrate interaction; X-ray crystallography; peptide probes | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-2481-4 | isbn_softcover | 978-1-0716-2483-8 | isbn_ebook | 978-1-0716-2481-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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