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Titlebook: Histology Protocols; Tim D. Hewitson,Ian A. Darby Book 2010 Humana Press 2010 Histochemistry.Histological material.Imaging techniques.Labo

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發(fā)表于 2025-3-21 18:56:24 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)Histology Protocols
編輯Tim D. Hewitson,Ian A. Darby
視頻videohttp://file.papertrans.cn/428/427282/427282.mp4
概述Provides an easily accessible reference volume for histological techniques written by leading researchers in the field.Details the techniques for investigating the expression of many different types o
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Histology Protocols; Tim D. Hewitson,Ian A. Darby Book 2010 Humana Press 2010 Histochemistry.Histological material.Imaging techniques.Labo
描述Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4–7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry,histologicalstaining (Chapters10and11),aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapt
出版日期Book 2010
關(guān)鍵詞Histochemistry; Histological material; Imaging techniques; Laboratory; Pathogenesis; Staining techniques;
版次1
doihttps://doi.org/10.1007/978-1-60327-345-9
isbn_softcover978-1-4939-5694-4
isbn_ebook978-1-60327-345-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2010
The information of publication is updating

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Book 2010ine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this
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Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Reer analysis of gene expression using real-time PCR. Microdissection of glomeruli from archival renal biopsy sections was carried out using the PALM Microbeam UV laser system from P.A.L.M. Technologies.
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High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopyed: (1) high-pressure freezing followed by freeze-substitution and immunogold labeling and (2) chemical fixation followed by freeze-substitution and immunogold labeling. Both methods have advantages and disadvantages that influence their utility in a given study design.
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Active Staining of Mouse Embryos for Magnetic Resonance Microscopymethods that allow one to balance the fixation, which reduces the nuclear magnetic resonance (NMR) signal, with the enhancement of signal derived from the reduction in T1. Methods are included to cover the ranges of embryonic specimens from E10.5 through E19.5.
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1064-3745 s for investigating the expression of many different types oSomuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. H
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Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cordd tissue sections following identification using retrograde tracing. The methods are broadly applicable to other cell types and can be combined with multiple label immunohistochemistry to study cellular constituents or with subsequent DAB staining to produce a permanent mount.
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