Titlebook: Histochemistry of Single Molecules; Methods and Protocol Carlo Pellicciari,Marco Biggiogera Book 2017 The Editor(s) (if applicable) and The

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Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in theminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.

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Histochemical Demonstration of Tripeptidyl Aminopeptidase Is for chromogenic and fluorogenic histochemical demonstration of tripeptidyl aminopeptidase I (TPPI), a protease that is crucial for neuronal functions. The procedures are based on newly synthesized substrates for TPPI—glycyl-.-prolyl-.-metionyl-5-chloro-1-anthraquinonylhydrazide (GPM-CAH) and glycy

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Lectin Histochemistry: Historical Perspectives, State of the Art, and the Futureliving organisms. Their precise functions are as yet under-explored and incompletely understood but they are clearly involved, through recognition of their binding partners, in a myriad of biological mechanisms involved in cell identity, adhesion, signaling, growth regulation, in health and disease.

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Panther

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The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epitheliumese glycoconjugates are associated with the entry of cells into apoptosis. A widely used technique for the detection of apoptotic cell death is TUNEL. In this chapter, we study the sensitivity of both techniques to identify apoptotic cells in the testis of photo-inhibited Syrian hamster.

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Agronomy

Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brains of the hippocampus and the olfactory bulb. In this chapter, we describe the procedures aimed to investigate adult neurogenesis in the murine brain. Specifically, we explain how to inject into animals exogenous markers of proliferation (i.e., BrdU) and prepare brain tissues to perform immunofluores

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