Titlebook: Histamine Receptors as Drug Targets; Ekaterini Tiligada,Madeleine Ennis Book 2017 Springer Science+Business Media, LLC, part of Springer N

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Evaluation of Histidine Decarboxylase (HDC) Activity and Human Histamine Receptor 3 (H3R) and HDC ms. These are a radiolabeled method for HDC activity measurement and quantitative real-time polymerase chain reaction (qPCR) methods for quantification and transcript analyses of human HDC and histamine receptor type 3 (H.R).

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Assessment of the Efficacy of Histamine Receptor Antagonists in Murine Models of Atopic Dermatitis,titis-like skin lesions (NC/Nga mice) and a protein-induced mouse model of atopic dermatitis (ovalbumin model) and discusses the role of the histamine receptor (HR) H.R and H.R antagonists in these models.

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Methodological Approaches to Study Histamine Pharmacology in the Kidney: From Cell Culture and Cellps taken to obtain a suitable model to study histamine pharmacology in renal cells, starting from the cell isolation to the morpho-functional assays. The in vitro system herein reported mostly reflects the in vivo situation as demonstrated by our previously published data.

GROG

Methodological Approaches to Investigate the Effects of Histamine Receptor Targeting Compounds in Pstamine receptor ligands in breast cancer, which could originate advances in novel therapeutics to treat this disease. This chapter also describes the gold standard radiometric techniques to evaluate the activity of histamine metabolizing enzymes in breast cancer specimens.

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Ligand-Binding Kinetics on Histamine Receptors,so known as residence time) are thought to be more important predictors of drug in vivo efficacy. This chapter provides the theoretical background on ligand-binding kinetics and several experimental approaches to determine the target residence time of antihistamines on histamine receptors.

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Methods to Study the Molecular Pharmacology of the Histamine H4 Receptor,igands, which can initiate differential efficacies in various GPCR responses. This chapter will provide an overview of commonly applied methods used to elucidate the molecular pharmacological aspects of the H.R from receptor-ligand-binding interactions to downstream gene transcription.

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Histamine and the Kidney: In Vivo Animal Models,e of histamine and its receptors in renal (patho)-physiology and to validate histamine receptors as new pharmacological targets in renal diseases. Within this chapter a suitable in vivo model to study diabetic nephropathy and some of the most important parameters to monitor the functional and morphological integrity of the kidney are described.