Titlebook: High-Speed Atomic Force Microscopy in Biology; Directly Watching Dy Toshio Ando Book 2022 Springer-Verlag GmbH Germany, part of Springer Na

悠然

Canonical Motor Proteinson HS-AFM images. Nevertheless, few motor proteins have yet been imaged with HS-AFM. This chapter focuses on HS-AFM studies on canonical motor proteins, myosin V and F.-ATPase. HS-AFM studies on noncanonical motor proteins are described in other chapters.

ALIAS

Molecular Chaperoneslar chaperones that promote proper folding of nascent polypeptides by facilitating folding and preventing misfolding and aggreagation. However, properly folded proteins are often subjected to several types of stress, resulting in misfolding and aggregation.

Palliation

Interactive HS-AFM (iHS-AFM)ranging from a simple system to a sophisticated system containing a haptic device. Thus far, only simple techniques have been incorporated into HS-AFM that can be installed by software modifications of the operating system. Although interactive HS-AFM (iHS-AFM) has been used only in a few cases, interesting results have been provided.

intimate

隱士

hair-bulb

Substrate Surfaces not strongly interact with a surface, which could interfere with their function. Nevertheless, when the interaction is too weak, the molecules move too fast on the surface to be clearly imaged even with HS-AFM.

有機(jī)體

Tip-Scanning HS-AFMis restriction is more rigorous in HS-AFM. In contrast, tip-scanning (stationary sample) AFM has no such restriction. There is an additional advantage: a tip-scanning AFM system can be easily combined with other systems such as optical microscopes.

enchant

Interactive HS-AFM (iHS-AFM)formation on these objects that cannot be attained by imaging alone. Sample manipulation techniques have been introduced to conventional AFM systems, ranging from a simple system to a sophisticated system containing a haptic device. Thus far, only simple techniques have been incorporated into HS-AFM

禮節(jié)

Influence of Tip–Sample Interactions on Specimensof the possible influences of tip–sample contact on the structure and the functional activity of the protein under study. Although the degree of this influence depends on the sample fragility, it is always good to know how large influences can occur quantitatively, depending on imaging conditions.

PAEAN