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Titlebook: High-Resolution Imaging of Cellular Proteins; Methods and Protocol Steven D. Schwartzbach,Omar Skalli,Thomas Schikors Book 2016 Springer Sc

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書目名稱High-Resolution Imaging of Cellular Proteins
副標(biāo)題Methods and Protocol
編輯Steven D. Schwartzbach,Omar Skalli,Thomas Schikors
視頻videohttp://file.papertrans.cn/427/426726/426726.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: High-Resolution Imaging of Cellular Proteins; Methods and Protocol Steven D. Schwartzbach,Omar Skalli,Thomas Schikors Book 2016 Springer Sc
描述.This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful .Methods in Molecular Biology series. format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field..
出版日期Book 2016
關(guān)鍵詞molecular tool box; epitope tagged proteins; polyclonal antibodies; anti-peptide antibodies; fluorescent
版次1
doihttps://doi.org/10.1007/978-1-4939-6352-2
isbn_softcover978-1-4939-8173-1
isbn_ebook978-1-4939-6352-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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hlich war das Bestreben, die Bewegung von K?rpern zu beschreiben, vor mehr als 400 Jahren die Geburtsstunde der Physik..Die Bewegung und die damit zusammenh?ngenden Konzepte der Kraft und der Masse bilden den Gegenstand der Mechanik. Im Rahmen der Mechanik werden wir uns zun?chst der Kinematik zuwen
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Harumi Harada,Ryuichi Shigemotontalphysik.Erweitert und verbessert durch Einbezug moderner .Das Arbeitsbuch zu ?Physik - für Studierende der Naturwissenschaften und Technik“ von Paul A. Tipler und Gene Mosca enth?lt alle Aufgaben der achten deutschsprachigen Ausgabe sowie deren ausführliche L?sungen..Mit über 1200 Aufgaben - daru
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Expression of Epitope-Tagged Proteins in Mammalian Cells in Culturetein of interest. Thus, only proteins for which antibodies were available could be visualized. Epitope tagging allows the detection of all proteins with existing sequence information, irrespective of the availability of antibodies directed against them. This technique involves the generation of DNA
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Antibody Production with Synthetic Peptidesclonal antibodies routinely with titers higher than 20,000. Peptide antigens do not function as immunogens unless they are conjugated to proteins. Production of high quality antipeptide antibodies is dependent upon peptide sequence selection, the success of peptide synthesis, peptide–carrier protein
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Production and Purification of Polyclonal Antibodieslls in response to an immunogen. Polyclonal antibodies raised against an antigen recognize multiple epitopes on a target molecule, which results in a signal amplification in indirect immunoassays including immune-electron microscopy. In this chapter, we present a basic procedure to generate polyclon
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