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Titlebook: Heterologous Gene Expression in E.coli; Methods and Protocol Nicola A. Burgess-Brown Book 2017 Springer Science+Business Media LLC 2017 Pro

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發(fā)表于 2025-3-21 18:51:52 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Heterologous Gene Expression in E.coli
副標(biāo)題Methods and Protocol
編輯Nicola A. Burgess-Brown
視頻videohttp://file.papertrans.cn/427/426032/426032.mp4
概述Includes cutting-edge methods and protocols for the study of E. coli gene expression.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from t
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Heterologous Gene Expression in E.coli; Methods and Protocol Nicola A. Burgess-Brown Book 2017 Springer Science+Business Media LLC 2017 Pro
描述This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput? cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Heterologous Gene Expression in E. coli: Methods and Protocols?.serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism..
出版日期Book 2017
關(guān)鍵詞Protein purification; Recombinant proteins; Escherichia coli; Structural biology; High-throughput clonin
版次1
doihttps://doi.org/10.1007/978-1-4939-6887-9
isbn_softcover978-1-4939-8328-5
isbn_ebook978-1-4939-6887-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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Natalie J. Saez,Ben Cristofori-Armstrong,Raveendra Anangi,Glenn F. King) because of hemodynamic instability or oxygenation problems. Therefore, evaluating the effectiveness of any pre-treatment with a potent organ protective intervention is extremely difficult in most patients. In contrast, cardiac surgery with cross-clamping of the ascending aorta results in predictab
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) because of hemodynamic instability or oxygenation problems. Therefore, evaluating the effectiveness of any pre-treatment with a potent organ protective intervention is extremely difficult in most patients. In contrast, cardiac surgery with cross-clamping of the ascending aorta results in predictab
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Benjamin Strutton,Stephen R. P. Jaffé,Jagroop Pandhal,Phillip C. WrightNO) synthesis [5], inhibition of ATP-dependent potassium channels [6] and restoration of adrenergic receptor sensitivity [7]. As a consequence, low doses of vasopressin analogs have been shown to increase mean arterial pressure (MAP) in catecholamine-resistant septic shock in numerous experimental [
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1064-3745 inning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism..978-1-4939-8328-5978-1-4939-6887-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
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