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Titlebook: Heterologous Expression of Membrane Proteins; Methods and Protocol Isabelle Mus-Veteau Book 2022Latest edition The Editor(s) (if applicable

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書目名稱Heterologous Expression of Membrane Proteins
副標(biāo)題Methods and Protocol
編輯Isabelle Mus-Veteau
視頻videohttp://file.papertrans.cn/427/426031/426031.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Heterologous Expression of Membrane Proteins; Methods and Protocol Isabelle Mus-Veteau Book 2022Latest edition The Editor(s) (if applicable
描述This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using .E. coli. as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and up-to-date, .Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition. serves as an ideal guide f
出版日期Book 2022Latest edition
關(guān)鍵詞Bacterial host models; Protein expression; Production systems; Structural studies; Protein purification
版次3
doihttps://doi.org/10.1007/978-1-0716-2368-8
isbn_softcover978-1-0716-2370-1
isbn_ebook978-1-0716-2368-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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ith which to study molecular recognition of proteins and non-proteins. Such libraries have been used extensively to define the specificity of protein/protein, protein/RNA, protein/peptide, and RNA/small molecule interactions.
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Landon Haslem,Marina Brown,Xin A. Zhang,Jennifer M. Hays,Franklin A. Haysmunity and clinical studies have changed the clinical perspective on late-life depression, not just complemented the extant studies of 10 years ago. In this review, I will concentrate upon those recent studies that have direct clinical relevance to the care of the depressed older adult.
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Hoor Ayub,Michelle Clare,Luke Broadbent,John Simms,Alan D. Goddard,Alice J. Rothnie,Roslyn M. Bill activity in Western Europe and the United States, particularly in the areas of health, family maintenance, and pension provision. In all areas of social welfare, legislative and market-based strategies are reconsidering the “artificial separation between ‘public’ and ‘private’” (Rainwater & Rein, 1983, p. 112).
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