Titlebook: Herpes Simplex Virus; Methods and Protocol Russell J. Diefenbach,Cornel Fraefel Book 2020Latest edition Springer Science+Business Media, LL

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Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for Use in High-Throughput his powerful approach has been especially useful for revealing the global genetic diversity that exists within and between strains of each virus species. However, most early methods for high-throughput sequencing required the input of abundant viral genomic DNA to enable the successful production of

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META

樹木心

Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivationeactivation. While several alternative models are available, including infections of live animal or explanted ganglia, these are complicated by the presence of multiple cell types, including immune cells, and difficulties in manipulating the neuronal environment. The highly pure neuron culture syste

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Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry, to analyze small entities such as bacteria, let alone viruses, owing to the 0.5?μm resolution of most instruments. To circumvent this limitation, some laboratories decorate pathogens with antibodies or nanoparticles. Our laboratory instead exploits an alternative approach that relies on the stainin

CLEAR

OREX

Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot,apid virus blotting immunoassay to define conformational changes with a panel of monoclonal antibodies to distinct sites across a viral glycoprotein. This dot blot technique has been utilized to define low pH-triggered changes in the prefusion form of the herpesviral fusogen gB. At pH of <6.2 there

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bromide