Titlebook: Hepatic Stem Cells; Methods and Protocol Naoki Tanimizu Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 biliar

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Induction of Functional Hepatocytes from Human iPSCsed liver progenitor cells, liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs). We also establish long-term two-dimensional co-culture system to induce functional hepatocytes from iPSC-derived liver cells.

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Culture System of Bile Duct-Like Cystic Structures Derived from Human-Inducible Pluripotent Stem Celre thought to be an important source of regenerative medicine because of their pluripotency and self-renewal ability. Although the liver has high regeneration ability, continuous death of hepatocytes due to chronic inflammation leads to liver cirrhosis and liver carcinoma. With regard to such seriou

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Identification and Isolation of Clonogenic Cholangiocyte in Mouselangiocytes from mouse. To study the heterogeneity of cholangiocytes, we used flow cytometry-based cell sorting to isolate different subsets of cholangiocytes. Organoid-forming efficiencies from sorted single cells are compared within different cholangiocyte populations to identify clonogenic cholangiocytes.

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Analysis for the Heterogeneity of Liver Progenitor Cellss. We have developed a new technique by combining genetic lineage tracking and three-dimensional imaging methods. The data obtained can be used in statistical analysis to quantitatively investigate the mechanisms underlying the heterogeneity of hepatic progenitor cells.

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Generation of Hepatic Tissue Structures Using Multicellular Spheroid Cultureg a structural feature of hepatic cord to represent further hepatic functions. Here we introduce a unique method to engineer the microarchitectures in the MCSs by formation of void spaces or filling of extracellular matrices (ECMs).

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Pengyu Huang,Lulu Sun,Ludi Zhang,Lijian Huiiling metrics proposed in the last two decades, including MUC, B-cubed, CEAF and BLANC. We will give illustrative examples to show how they are computed, and the scenarios under which they are intended to be used. We will present their strengths and weaknesses, and clarify some misunderstandings of the metrics found in the recent literature.

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