Titlebook: Hemostasis and Thrombosis Protocols; David J. Perry,K. J. Pasi Book 1999 Springer Science+Business Media New York 1999

CRUST

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HALL

Amplification of DNA and RNA by PCRof the chapters employ the technique at some point to amplify specific DNA or RNA sequences. This chapter, therefore, provides a brief outline of the techniques and methods for the amplification of both DNA and RNA. It should be remembered that no single protocol will be suitable for all PCR reactio

NIB

Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beadsf the amplification primers and as a result becomes incorporated into PCR product during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobi

Maximizer

Vulnerary

Detection of DNA by Silver Stainingtages over conventional staining methods, including the ability to detect very small amounts of material, while avoiding the hazards of other detection systems, such as ethidium bromide or radio-isotopes.

Gustatory

Promoter Studies in Hemostasised the production of cloned genomic material encompassing the putative promoter regions of genes involved in the hemostatic process. These recombinant vectors provide the raw material for reporter gene studies and DNA footprinting analysis; two of the three most frequently used in vitro procedures t

Brain-Waves

Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoretions and polymorphisms. The most frequently used screening methods for mutation hunting in bleeding disorders are denaturing gradient gel electrophoresis (DGGE) (.), single-strand conformation polymorphism (SSCP) (.), and chemical mismatch cleavage (.). This chapter gives an introduction to the the

Offset

致命

Endearing