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Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 20051st edition Humana Press 2005 Antigen.Termination.antibody.biolog

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發(fā)表于 2025-3-21 16:41:37 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Handbook of ELISPOT
副標(biāo)題Methods and Protocol
編輯Alexander E. Kalyuzhny
視頻videohttp://file.papertrans.cn/422/421201/421201.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 20051st edition Humana Press 2005 Antigen.Termination.antibody.biolog
描述In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.
出版日期Book 20051st edition
關(guān)鍵詞Antigen; Termination; antibody; biology; cell; chemistry; cytokine; cytokines; development; immune response; i
版次1
doihttps://doi.org/10.1385/1592599036
isbn_softcover978-1-61737-588-0
isbn_ebook978-1-59259-903-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2005
The information of publication is updating

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沙發(fā)
發(fā)表于 2025-3-21 22:44:36 | 只看該作者
Alvin H. Strelnick,Margaret Gilpinexperienced scientists as well as newcomers to the field an overview over the major standardization issues for each step of the protocol. Guidelines are given on how to validate the ELISPOT performance.
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Mohd Farid Ahmad,Muhammad Syahmi Hishamuddinymphocytes with antigens. In rhesus macaques immunized with an HIV envelope peptide cocktail vaccine, the DC-ELISPOT protocol enabled more accurate enumeration of the cellular immune responses, as antigen-specific inteferon-γ-producing cells, with up to 18-fold increase in detection sensitivity comp
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Urban Identity and the Atlantic Worldoteins of myelin: myelin basic protein and proteolipid protein. Our goal was to use the enzyme-linked immunospot assay to perform comprehensive epitope mapping in relapsing-remitting MS patients in a longitudinal study to help define the role of myelin responses in disease progression.
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發(fā)表于 2025-3-23 07:55:35 | 只看該作者
Standardization and Validation Issues of the ELISPOT Assayexperienced scientists as well as newcomers to the field an overview over the major standardization issues for each step of the protocol. Guidelines are given on how to validate the ELISPOT performance.
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