Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 2018Latest edition Springer Science+Business Media, LLC, part of Spri

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書目名稱Handbook of ELISPOT影響因子(影響力)




書目名稱Handbook of ELISPOT影響因子(影響力)學(xué)科排名




書目名稱Handbook of ELISPOT網(wǎng)絡(luò)公開度




書目名稱Handbook of ELISPOT網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Handbook of ELISPOT被引頻次




書目名稱Handbook of ELISPOT被引頻次學(xué)科排名




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Mastering the Computational Challenges of Elispot Plate Evaluation, addressing the analysis step, the plate evaluation. Comparing that fact with the vast amount of analysis advise available for other single cell immune assay, for example, intracellular cytokine staining, the overall impression may be that Elispot evaluation is just simple enough to not require exte

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Automatic Search of Spots and Color Classification in ELISPOT Assay,their morphological variations make it difficult developing a reliable software application. An image recognition method allowing the automatic detection and classification of round objects (spots) on ELISPOT images independently of the registration conditions was developed. The emphasis is done on

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,Four Color ImmunoSpot? Assays for Identification of Effector T-Cell Lineages,FN-γ in response to antigen exposure, these assays permit the measurement of the frequency of antigen-specific T cells among white blood cells. These assays therefore are well suited to assess clonal expansions, that is, whether a (Th1) T cell response has been induced to an antigen in a test subjec

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Detection of Cross-Reactive B Cells Using the FluoroSpot Assay,ingle antigen. By reversing the B cell ELISpot and using anti-IgG capture antibodies instead of coated antigen, the specificity of antibodies secreted by B cells can be defined using soluble tagged antigen for detection. When combining this approach with fluorescent detection of the antigen in a B c

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,Multiplex ImmunoSpot? Assays for the Study of Functional B Cell Subpopulations,of effector molecules such as cytokines, chemokines, and granzyme. B cell subpopulations releasing such effector molecules have been implicated in immunobiology and a number of diseases..Unlike antigen-specific T cells that can be identified by multimer staining, and then counter-stained to define T

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Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpotUnique single cell resolution along with high throughput potential sets ELISPOT apart from flow cytometry, ELISA, microarray- and bead-based multiplex assays. The necessity to unambiguously identify individual T and B cells that do, or do not co-express certain analytes, including polyfunctional cyt

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,Multi-Color FLUOROSPOT Counting Using ImmunoSpot? Fluoro-X? Suite, have recently become a major new avenue of development of ELISPOT technology. Advances in assay techniques and the availability of commercial test kits stimulated development of multi-color FLUOROSPOT data analysis platforms. The ImmunoSpot. Fluoro-X? Software Suite was developed by CTL as an integ