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Titlebook: HIV Protocols ; Vinayaka R. Prasad,Ganjam V. Kalpana Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclus

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發(fā)表于 2025-3-21 17:46:19 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)HIV Protocols
編輯Vinayaka R. Prasad,Ganjam V. Kalpana
視頻videohttp://file.papertrans.cn/421/420195/420195.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: HIV Protocols ; Vinayaka R. Prasad,Ganjam V. Kalpana Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclus
描述This fourth edition volume expands on the previous editions with discussions on the latest methodologies to study HIV, live cell imaging, HIV cure, new modifications to the viral RNA that impacts HIV biology, and new types of intracellular compartments. The chapters in this book are organized into seven parts and cover topics such as HIV latency reactivation via single molecule RNA detection; T cell responses; new and efficacious anti-HIV CAR T cells; analysis of mucosal HIV infection; analysis of 3D brain organoids to study neuro AIDS; and the transfer of antibodies across the blood brain barrier. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and authoritative, .HIV Protocols, Fourth Edition. is a valuable resource for all preclinical HIV-1 researchers looking to learn more about this important and advancing field.?.
出版日期Book 2024Latest edition
關(guān)鍵詞protease activation; T Cell Responses; Resting CD4+ T Cells; Single-cell imaging RNA-FISH; HIV neuropath
版次4
doihttps://doi.org/10.1007/978-1-0716-3862-0
isbn_softcover978-1-0716-3864-4
isbn_ebook978-1-0716-3862-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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發(fā)表于 2025-3-21 21:11:12 | 只看該作者
Single-Cell Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolunted information about cell-to-cell variability in reactivation. Here, we describe the single-cell single-molecule RNA-FISH (smRNA-FISH) method to visualize HIV-1 gag RNA combined with the immunofluorescence (IF) method to detect Gag protein to characterize the reactivated cells. This method allows
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發(fā)表于 2025-3-22 03:00:48 | 只看該作者
Single-Virion Analysis: A Method to Visualize HIV-1 Particle Content Using Fluorescence Microscopyanalysis was first developed to study HIV-1 RNA genome packaging. In this assay, HIV-1 unspliced RNA is labeled with a fluorescently tagged RNA-binding protein (protein A) and some of the Gag proteins are labeled with a different fluorescent protein (protein B). Using fluorescence microscopy, HIV-1
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發(fā)表于 2025-3-23 03:34:18 | 只看該作者
The Glaciers of Equatorial East Africanted information about cell-to-cell variability in reactivation. Here, we describe the single-cell single-molecule RNA-FISH (smRNA-FISH) method to visualize HIV-1 gag RNA combined with the immunofluorescence (IF) method to detect Gag protein to characterize the reactivated cells. This method allows
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發(fā)表于 2025-3-23 08:44:18 | 只看該作者
https://doi.org/10.1007/978-0-387-76700-0analysis was first developed to study HIV-1 RNA genome packaging. In this assay, HIV-1 unspliced RNA is labeled with a fluorescently tagged RNA-binding protein (protein A) and some of the Gag proteins are labeled with a different fluorescent protein (protein B). Using fluorescence microscopy, HIV-1
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