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Malnutrition

書目名稱Golden Gate Cloning影響因子(影響力)




書目名稱Golden Gate Cloning影響因子(影響力)學(xué)科排名




書目名稱Golden Gate Cloning網(wǎng)絡(luò)公開度




書目名稱Golden Gate Cloning網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Golden Gate Cloning被引頻次




書目名稱Golden Gate Cloning被引頻次學(xué)科排名




書目名稱Golden Gate Cloning年度引用




書目名稱Golden Gate Cloning年度引用學(xué)科排名




書目名稱Golden Gate Cloning讀者反饋




書目名稱Golden Gate Cloning讀者反饋學(xué)科排名

有發(fā)明天才

Use of a Golden Gate Plasmid Set Enabling Scarless MoClo-Compatible Transcription Unit Assembly,erate transcription units. Typically, type IIS enzymes are used, which generate four nucleotide overhangs. This results in small scar sequences in hierarchical DNA assemblies, which can affect the functionality of transcription units. However, there are enzymes that generate three nucleotide overhan

半球

inveigh

Validation of Golden Gate Assemblies Using Highly Multiplexed Nanopore Amplicon Sequencing,libraries, resulting in assemblies of high combinatorial complexity where sequencing again becomes mandatory. Here, we present a detailed protocol for the use of a highly multiplexed dual barcode amplicon sequencing using the Nanopore sequencing platform for in-house sequence validation. The workflo

Ptosis

Modular Golden Gate Assembly of Linear DNA Templates for Cell-Free Prototyping,tion of a repressor–promoter pair. By eliminating time-consuming transformation and cloning steps in cells and by taking advantage of modular cloning toolboxes, our cell-free prototyping workflow can produce data for large numbers of new assembled constructs within a single day.

convert

convert

BIDE

Lumbar-Spine

Rosemarie Klemm,Dietrich D. Klemming the type of part that needs to be cloned, (2) designing primers for amplification, (3) performing polymerase chain reaction (PCR) amplification, (4) cloning of the fragments using Golden Gate cloning, and finally (5) sequencing of the part. For large standard parts, it is preferable to first clo

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