Titlebook: Glycovirology Protocols; Richard J. Sugrue Book 2007 Humana Press 2007 Hepatitis.Polyacrylamid-Gelelektrophorese.antibody.biology.infectio

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Analysis of Glycoproteins of Viruses in the Family ,,g protective immunity. The glycoproteins are modified by N-linked glycosylation and accumulate in the Golgi complex where virions mature and bud. In this chapter, we describe the methods that have been used in our laboratory for the study of the glycoproteins of Bunyamwera virus, the prototype of th

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Secretion of the Respiratory Syncytial Virus Fusion Protein From Insect Cells Using the Baculoviruse (GST)-tagged proteins. The sequence covering the F. subunit (GST-F2), and a truncated form of the F protein in which the transmembrane domain was removed (GST-F./F., were cloned into the baculovirus pAcSecG2T secretory vector. These virus sequences also had the endogenous virus signal sequence rem

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Characterization of the Dengue Virus Envelope Glycoprotein Expressed in ,,yeast, ., using a secretory vector. A truncated form of the E protein in which the transmembrane domain was deleted was secreted successfully into the culture medium. The E protein was also co-expressed with C and prM proteins using a non-secretory yeast vector. The co-expression of C, prM and E pro

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Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coroform SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy.

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1064-3745 chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.978-1-61737-664-1978-1-59745-393-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

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