Titlebook: Glycoanalysis Protocols; Elizabeth F. Hounsell Book 1998Latest edition Humana Press 1998

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書目名稱Glycoanalysis Protocols網(wǎng)絡(luò)公開度學科排名




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Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography,e cellular phenomena, assessment of the structure of sugar chains is indispensable. However, it is difficult to elucidate the structures of cell surface oligosaccharides because of two technical problems. First is the difficulty in fractionating various oligosaccharides heterogeneous in the number,

悠然

Meditative

occurrence

occurrence

Analysis of ,- and ,-Glycans by Pyridylamination,thod has been applied to structure analyses of glycans including glycosidase digestion (.), 2D-mapping by various kinds of high-performance liquid chromatography (HPLC) (.), partial acetolysis (.), Smith degradation (.), methylation analysis (.), nuclear magnetic resonance (.), mass spectrometry (.)

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Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins, spectroscopy, and rarely nuclear magnetic resonance spectroscopy. These methods required relatively large amounts of materials (micromoles), specialized training and experience, and in some cases, significant capital equipment outlays. Because of these restrictions, convenient carbohydrate analysts

surrogate

反饋

The Use of Cell and Organ Culture for the Study of Secreted Mucins,e of human colonic cell lines as a model of the adenoma-carcinoma sequence is of particular interest because it allows the changes in expression of mucins to be studied during the development and progression of disease (.,.). Recently, the importance of proliferation, differentiation, and apoptosis