Titlebook: Glyco-Engineering; Methods and Protocol Alexandra Castilho Book 2015 Springer Science+Business Media New York 2015 Carbohydrate composition

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Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in , biotechnologically relevant host .. Here we provide insight on how to select the best strains and plasmids. We also describe methods for determination of glycan expression and assembly, protein glycosylation using western blot, and preparation of samples for mass spectrometry.

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Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in ,new possibilities for glyco-engineering have arisen, and a wide array of glycans can be designed and potentially transferred to target proteins in the biotechnologically relevant host .. Here we provide insight on how to select the best strains and plasmids. We also describe methods for determinatio

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Progress in Yeast Glycosylation Engineering used as model organisms to facilitate our understanding of such features and processes. To this end, a large number of powerful genetic tools have been developed to investigate and manipulate these organisms. Going hand-in-hand with these genetic tools is the ability to efficiently scale up the fer

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Protein Production with a , OCH1 Knockout Strain in Fed-Batch Mode the target product describes a glycoprotein, an α-1,6-mannosyltransferase located in the Golgi apparatus of ., called OCH1, triggers hypermannosylation of the recombinant protein which significantly impedes following unit operations and hampers biopharmaceutical product applications. A knockout of

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