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Titlebook: Glucose Transport; Methods and Protocol Karin Lindkvist-Petersson,Jesper S‘Hansen Book 2018 Springer Science+Business Media LLC 2018 GLUTs.

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書目名稱Glucose Transport
副標題Methods and Protocol
編輯Karin Lindkvist-Petersson,Jesper S‘Hansen
視頻videohttp://file.papertrans.cn/388/387051/387051.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Glucose Transport; Methods and Protocol Karin Lindkvist-Petersson,Jesper S‘Hansen Book 2018 Springer Science+Business Media LLC 2018 GLUTs.
描述This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling strategies combined with advanced fluorescence microscopy methods, and tracking GLUT2 translocation by live cell imaging. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Glucose Transport: Methods and Protocols. is a valuable resource that helps researchers with determining specific roles of different GLUTs in various organisms..
出版日期Book 2018
關鍵詞GLUTs; SGLTs; yeast; FRET-Based Assay; hexose transport; proteins
版次1
doihttps://doi.org/10.1007/978-1-4939-7507-5
isbn_softcover978-1-4939-8506-7
isbn_ebook978-1-4939-7507-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2018
The information of publication is updating

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Karin Lindkvist-Petersson,Jesper S‘HansenIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
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Jeffrey A. Cardille,Monica G. Turnerput screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar transporters heterologously expressed in it.
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