Titlebook: Glioblastoma; Methods and Protocol Dimitris G.‘Placantonakis Book 2018 Springer Science+Business Media, LLC 2018 xenografts.GBM cancer stem

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Hong Kong Architecture 1945-2015issection (LCM) offers a unique opportunity to study cells in their topological contexts. This chapter focuses on the preparation of LCM membrane slides, tissue staining and laser microdissection of cells of interest from frozen or formalin-fixed, paraffin-embedded glioblastoma tissue.

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Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF,ype. The variability in blood perfusion and oxygen tension within tumors suggests that ambient pH values fluctuate across different tumor territories. This chapter describes a detailed protocol for measuring intracellular pH in patient-derived GBM cells in vitro, using the fluorescent pH sensitive dye BCECF.

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978-1-4939-8536-4Springer Science+Business Media, LLC 2018

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Glioblastoma978-1-4939-7659-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Hom?opathie - die Fakten [unverdünnt]ent of disease. The goal is to use high-throughput sequencing to identify specific variants that drive tumorigenesis within each individual’s tumor genomic profile. The significance of copy number and structural variants in glioblastoma makes it essential to broaden the search beyond oncogenic singl

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https://doi.org/10.1007/978-3-662-55549-1 glioblastoma molecular profiles. The procedure spans four days, and can be performed by a single laboratory technician. Starting with as little as 250 ng of DNA input, this method allows the flexibility to begin with DNA derived from either formalin-fixed, paraffin-embedded (FFPE) or fresh tissue a

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https://doi.org/10.1007/978-3-642-77601-4 or by tumorsphere culture in suspension. Here, we provide a detailed protocol for establishing patient-derived tumorsphere cultures. Such cultures are enriched for GBM stem cells (GSCs) and can be used to generate orthotopic tumor xenografts in the brain of immunocompromised mice. We also point out

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Honda‘s Global Local Corporationcell sorting (FACS) using CD133 as a surface marker. The use of a directly conjugated antibody to an APC fluorophore against the CD133 molecule provides sufficient and clear detection of positive cells from the rest of the population. This strategy avoids an unnecessary secondary antibody incubation