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Titlebook: Germline Stem Cells; Michael Buszczak Book 2017Latest edition Springer Science+Business Media New York 2017 Germline stem cell.primordial

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發(fā)表于 2025-3-28 15:13:43 | 只看該作者
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Per Andersson,Lars-Gunnar Mattsson specific regions in the gonads and can be identified because they uniquely express the . gene, which encodes a conserved regulator of translation. A method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in sit
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https://doi.org/10.1007/978-1-4471-0963-1 included the lack of specific markers and methods for lineage tracing of A. spermatogonia and their subsets. Immunolocalization of proteins in tissue sections has been a standard tool for the in situ identification and visualization of rare cellular subsets. However, these studies are limited by th
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https://doi.org/10.1007/978-1-4612-3712-9ell-specific gene and protein expression, as well as determine direct effects of signaling molecules, it is necessary to prepare enriched populations of germ cells and maintain them in culture for several hours to multiple days. The protocols in this chapter are designed to provide a guide for the i
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