Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2020 Springer Science+Business Media, LLC, part of Spring

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https://doi.org/10.1007/978-3-662-31645-0ation of VHH antibody yeast surface display libraries from immunized Alpacas and Lamas as well as the facile isolation of antigen-specific molecules in a convenient fluorescence-activated cell sorting (FACS)-based selection process.

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https://doi.org/10.1007/978-3-662-31644-3he techniques used to select target-binding ligands via this approach including the use of target-negative cells to deplete non-specific binders and avidity reduction to preferentially select high-affinity ligands.

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Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens,ion method, for our goal. In this book chapter, we describe the antibody selection process (panning) in solution and its advantages over panning on immobilized antigens. Detailed protocols on the panning procedure and the screening of monoclonal binders are given.

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