Titlebook: Genomics of Cereal Crops; Shabir Hussain Wani,Anuj Kumar Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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Virus Induced Gene Silencing: A Tool to Study Gene Function in Wheat,ansformation in a relatively short span of time as compared to other methods such as TILLING, EcoTILLING, and RNAi. In this chapter, we discussed the basics of posttranscriptional gene silencing and described step-by-step protocols for functional annotation of wheat genes using the barley stripe mos

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Common Genomic Tools and Their Implementations in Genetic Improvement of Cereals,insights into their complex genomes. Knowledge of genes can assist in manipulating their response toward stress and transferring important genes to other crop species via conventional breeding methods, marker assisted breeding or transgenic approaches for crop improvement. The present chapter provid

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Approaches of Single-Cell Analysis in Crop Improvement,ribed in this chapter. We also describe the batch effect correction in integrated omics data, single-cell trajectory and pseudotime analysis, GO enrichment, pathway analysis, and correlation analysis between single cell–based analysis and bulk analysis for crops. These approaches help in crop breedi

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Genome-Wide Association Study (GWAS) for Trait Analysis in Crops,it association. Still, challenges lie in detection of rare variants with small effect instead of ignoring during analysis which needs further improvement in computational efficiency and redesigning of statistical models.

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Tools and Techniques for Genomic Imprinting, and development. Here, we summarize the genomic imprinting mechanisms, several high-throughput strategies, data requirement, and statistical and computational tools to identify the novel imprinted genes.

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