Titlebook: Genomic Imprinting; Methods and Protocol Andrew Ward Book 2002 Humana Press 2002

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無(wú)法解釋

AMITY

In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs,CpGrich DNA can contribute to the effects of DNA methylation by formation of a repressive chromatin structure (.). In addition, selective DNA methylation is required to distinguish the effects of DNA methylation on transcription initiation and transcript elongation (.). Thus, there is a requirement

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In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation, either two silent alleles (., . and .) or two expressed alleles (., .., ., and .) (.,.–.). Although there may be exceptions (for instance, imprinted expression of .2 is maintained in .. embryos; ref. .), differential methylation is likely to be an important aspect of the imprinting mechanism that i

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Das Krankheitsbild Demenz verstehen,etween complex genomes by the use of subtractive hybridisation (1). This protocol for methylation-sensitive RDA (Me-RDA) describes adaptations to the original technique that, by the use of oligonucleotides for .

伸展

https://doi.org/10.1007/978-90-313-9801-0iteria for the identification of the parental origin of individual alleles and larger portions of the genome: DNA methylation is stably propagated in somatic cells during cell division, it is reversible, it may inactivate the target sequence, and male and female gametes have different methylation patterns (reviewed in ...).

極深

Identification of Imprinted Loci by Methylation,etween complex genomes by the use of subtractive hybridisation (1). This protocol for methylation-sensitive RDA (Me-RDA) describes adaptations to the original technique that, by the use of oligonucleotides for .