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Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under

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發(fā)表于 2025-3-21 19:11:19 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Genome Editing in Animals
副標題Methods and Protocol
編輯Izuho Hatada
視頻videohttp://file.papertrans.cn/383/382836/382836.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under
描述.This second edition provides new and updated protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the successful?.Methods in Molecular Biology.?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..?..Authoritative and cutting-edge,.?Genome Editing in Animals: Methods and Protocols, Second Edition .aims to be a?useful practical guide to researches to help further their study in this field.?.
出版日期Book 2023Latest edition
關(guān)鍵詞Zinc Finger Nucleases; TALEN; CRISPR/Cas9; Embryonic Stem Cells; knockout mice
版次2
doihttps://doi.org/10.1007/978-1-0716-3016-7
isbn_softcover978-1-0716-3018-1
isbn_ebook978-1-0716-3016-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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發(fā)表于 2025-3-21 22:50:12 | 只看該作者
Updated Overview of TALEN Construction Systems,mizable DNA-binding repeats and nuclease domain of FokI enzyme. Each DNA-binding repeat recognizes one base of double-strand DNA, and functional TALEN can be created by a simple modular assembly of these repeats. To easily and efficiently assemble the highly repetitive DNA-binding repeat arrays, var
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SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gin their causative genes. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is a flexible and efficient genome engineering technology widely used for researches and therapeutic applications which offers immense opportunity to treat genetic dis
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Generation of Genome-Edited Mice by Cytoplasmic Injection of CRISPR-Cas9 RNA,ion of genome-edited animals. The Cas9/guide RNA (gRNA) component can be introduced into zygotes in several ways. Here, we provide an instructional guide for the generation of knockout mice using cytoplasmic injection of in vitro transcribed Cas9 RNA and gRNA.
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Generation of Knock-In Mouse by Genome Editing,ryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome-editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an exam
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