Titlebook: Genetically Incorporated Non-Canonical Amino Acids; Methods and Protocol Yu-Hsuan Tsai,Simon J. Els?sser Book 2023 The Editor(s) (if applic

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書目名稱Genetically Incorporated Non-Canonical Amino Acids讀者反饋學(xué)科排名

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Engineering Homogeneous Photoactive Antibody Fragmentse describe a procedure for engineering photoactive antibody fragments that bind to their target antigen only after irradiation with 365?nm light. The procedure starts with identification of tyrosine residues in antibody fragments that are important for antibody–antigen binding and thus targets for r

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Repurposing Photosensitizer Proteins Through Genetic Code Expansion to Facilitate Photo-Biocatalysisalysts. Genetically encoded photo-redox cofactors endow photoenzymes with enhanced or novel activities that catalyze numerous transformations with high efficiency. Herein, we describe a protocol of repurposing photosensitizer proteins (.) through genetic code expansion to facilitate multiple photoca

SPASM

Genetic Encoding of a Fluorescent Noncanonical Amino Acid as a FRET Donor for the?Analysis of Deubiqing of proteins. These co-translational and internal fluorescent tags have been harnessed to establish genetically encoded F?rster resonance energy transfer (FRET) probes for studying protein structural changes and interactions. Here, we describe the protocols for site-specific incorporation of an a

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Creating Selenocysteine-Specific Reporters Using Inteins. These characteristics are attractive for designing highly active enzymes or extremely stable proteins and studying protein folding or electron transfer, to name a few. There are also 25 human selenoproteins, of which many are essential for our survival. The ability to create or study these selenop

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Genetically Encoded Noncanonical Amino Acids in Proteins to Investigate Lysine Benzoylationion and degradation. Histone lysine benzoylation is a recently identified epigenetic marker associated with active transcription, which has physiological relevance distinct from histone acetylation and can be regulated by debenzoylation of sirtuin 2 (SIRT2). Herein, we provide a protocol for the inc

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Semisynthesis of Glutamine-Methylated Proteins Enabled by Genetic Code Expansionelucidate the biological implications of this modification. Herein, we describe a protocol to generate histones with site-specific Gln methylation in a semisynthetic manner. Firstly, an esterified glutamic acid analogue (BnE) is genetically encoded into proteins by genetic code expansion with high e