Titlebook: Genetic Transformation Systems in Fungi, Volume 1; Marco A. van den Berg,Karunakaran Maruthachalam Book 2015 Springer International Publis

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Intestinal Fluid Handling: Secretion genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of ..

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https://doi.org/10.1007/978-1-4471-0471-1ng the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.

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Biolistic Transformation of , for Homoplasmic Mitochondrial Genome Transformantss located on the genome and many important metabolism functions associated with mitochondria, such as energy metabolism, generation of reactive oxygen species. Here, we describe a method to transform the mitochondrial genome of . by biolistic transformation.

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Use of the Biolistic Particle Delivery System to Transform Fungal Genomesng the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.

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Mediated Transformationzing the AMT process with new fungal species. The chapter also includes a highly efficient vector construction system based on Uracil Specific Excisions Reagent (USER) cloning and specific PCR generated building blocks, which can be combined ad hoc to create complex plasmids in a single cloning step.