Titlebook: Genetic Engineering of Microorganisms for Chemicals; Alexander Hollaender,Ralph D. DeMoss,Ralph S. Wolf Book 1982 Springer Science+Busines

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星球的光亮度

Detoxification

aneurysm

輕彈

Cloning Vectors Derived from Bacterial Plasmids,e number of naturally occuring plasmids has led to the conclusion that they have evolved through a process which can be referred to as “modular evolution”. In this process, various functional units (such as those involved in conjugal transfer, antibiotic resistance and colicin production) have been

得體

Expression of a Foreign Procaryotic Gene in ,,nsformation has lead to the development of a circular genetic map, the identification and approximate localization of the replication origin and terminus for the chromosome, as well as to studies of extrachromosomal inheritance (1,2). In recent years gene cloning in .. . has been accomplished by sev

得體

Fusion of Microbial Protoplasts: Problems and Perspectives, in the scheme of Figure 1. The rigid cell wall of bacteria, fungi, yeast, and algae (as well as of plant cells) can be removed without influencing the viability and integrity of the cell. The wall-less microorganism is surrounded by a plasma membrane, in hypertonic media it is spherical in shape, a

有助于

Strategies: Models and Problems,before any engineering is begun, we might take note of microbial enzymatic reactions at present in operation. What general patterns have been established by the more subtle pressures of evolution? Time is not available for an adequate survey, and a few examples from one important area must suffice:

forager

PANEL

Transformation of , Utilizing Recombinant Plasmid DNA, dependent on two different though related matters: an efficient transformation system for the organism and an appropriate vector. In addition, transformation in any organism involves two processes: a system which permits the uptake of DNA into the cell and the subsequent integration of the DNA into