找回密碼
 To register

QQ登錄

只需一步,快速開(kāi)始

掃一掃,訪問(wèn)微社區(qū)

打印 上一主題 下一主題

Titlebook: Generation of cDNA Libraries; Methods and Protocol Shao-Yao Ying Book 2003 Humana Press 2003

[復(fù)制鏈接]
查看: 31963|回復(fù): 61
樓主
發(fā)表于 2025-3-21 16:29:34 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Generation of cDNA Libraries
副標(biāo)題Methods and Protocol
編輯Shao-Yao Ying
視頻videohttp://file.papertrans.cn/383/382295/382295.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Generation of cDNA Libraries; Methods and Protocol Shao-Yao Ying Book 2003 Humana Press 2003
描述Since its invention and subsequent development nearly 20 years ago, po- merase chain reaction (PCR) has been extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current decoding and potential application of human genome information to genechips, there are urgent needs for identification of functional significance of these decoded gene sequences. Inherent in bringing these app- cations to fruition is the need to generate a complete and full-length cDNA library for potential functional assays of specific gene sequences. Generation of cDNA Libraries: Methods and Protocols serves as a laboratory manual on the evolution of generation of cDNA libraries, covering both ba- ground information and step-by-step practical laboratory recipes for which p- tocols, reagents, operational tips, instrumentation, and other requirements are detailed. The first chapter of the book is an overview of the basics of generating cDNA libraries, which include the following: (a
出版日期Book 2003
版次1
doihttps://doi.org/10.1385/1592593593
isbn_softcover978-1-61737-333-6
isbn_ebook978-1-59259-359-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2003
The information of publication is updating

書目名稱Generation of cDNA Libraries影響因子(影響力)




書目名稱Generation of cDNA Libraries影響因子(影響力)學(xué)科排名




書目名稱Generation of cDNA Libraries網(wǎng)絡(luò)公開(kāi)度




書目名稱Generation of cDNA Libraries網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書目名稱Generation of cDNA Libraries被引頻次




書目名稱Generation of cDNA Libraries被引頻次學(xué)科排名




書目名稱Generation of cDNA Libraries年度引用




書目名稱Generation of cDNA Libraries年度引用學(xué)科排名




書目名稱Generation of cDNA Libraries讀者反饋




書目名稱Generation of cDNA Libraries讀者反饋學(xué)科排名




單選投票, 共有 1 人參與投票
 

0票 0.00%

Perfect with Aesthetics

 

0票 0.00%

Better Implies Difficulty

 

1票 100.00%

Good and Satisfactory

 

0票 0.00%

Adverse Performance

 

0票 0.00%

Disdainful Garbage

您所在的用戶組沒(méi)有投票權(quán)限
沙發(fā)
發(fā)表于 2025-3-21 23:36:10 | 只看該作者
https://doi.org/10.1057/9780230316973protein. The basis of the widely used novel strategies for the generation of cDNA libraries are base pair complementarities, reverse transcription, and polymerase chain reactions. This chapter presents some general information on the principles of, biology behind, basic protocols of, and reagents us
板凳
發(fā)表于 2025-3-22 00:29:13 | 只看該作者
地板
發(fā)表于 2025-3-22 06:42:05 | 只看該作者
Definitions and Conversions of Unitsmprehensive analysis is most frequently carried out on a single-clone-for-single-gene basis, whereas gene discovery is done through the isolation of multiple cDNA clones for a single gene. Thus, current requirements for high-quality cDNA libraries suitable for comprehensive cDNA analysis are (1) hig
5#
發(fā)表于 2025-3-22 10:06:10 | 只看該作者
6#
發(fā)表于 2025-3-22 13:32:51 | 只看該作者
https://doi.org/10.1007/978-3-319-93236-1hods. A minimum of several thousand cells is needed for an acceptable quality of RNA extraction. Because of tissue heterogeneity, these methods usually provided neither reliable nor reproducible results. Unfortunately, it is impossible to collect adequate amounts of pure or homogeneous samples for t
7#
發(fā)表于 2025-3-22 17:06:39 | 只看該作者
8#
發(fā)表于 2025-3-22 23:20:23 | 只看該作者
Convex Bodies and Hypersurfaces structure at the 5′ end of eukaryotic mRNA to design novel methods for PCR-based cDNA library construction (.–.). Moreover, the strategy has been applied to identifying gene expression and isolating full-length cDNA (.–.). In our laboratory, the conventional and PCR-based strategies have been utili
9#
發(fā)表于 2025-3-23 01:46:13 | 只看該作者
10#
發(fā)表于 2025-3-23 07:57:59 | 只看該作者
 關(guān)于派博傳思  派博傳思旗下網(wǎng)站  友情鏈接
派博傳思介紹 公司地理位置 論文服務(wù)流程 影響因子官網(wǎng) 吾愛(ài)論文網(wǎng) 大講堂 北京大學(xué) Oxford Uni. Harvard Uni.
發(fā)展歷史沿革 期刊點(diǎn)評(píng) 投稿經(jīng)驗(yàn)總結(jié) SCIENCEGARD IMPACTFACTOR 派博系數(shù) 清華大學(xué) Yale Uni. Stanford Uni.
QQ|Archiver|手機(jī)版|小黑屋| 派博傳思國(guó)際 ( 京公網(wǎng)安備110108008328) GMT+8, 2025-10-6 14:51
Copyright © 2001-2015 派博傳思   京公網(wǎng)安備110108008328 版權(quán)所有 All rights reserved
快速回復(fù) 返回頂部 返回列表
高安市| 德惠市| 隆安县| 荥经县| 黄浦区| 红河县| 和静县| 峡江县| 茂名市| 临澧县| 即墨市| 盐城市| 新密市| 梅河口市| 大名县| 措美县| 郸城县| 苏尼特左旗| 贺州市| 山阴县| 东明县| 延长县| 霍邱县| 黄骅市| 石阡县| 顺平县| 尉氏县| 乐业县| 大理市| 都安| 常山县| 金沙县| 壤塘县| 怀安县| 嘉荫县| 金寨县| 西乌珠穆沁旗| 登封市| 鱼台县| 滁州市| 随州市|