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Titlebook: Gene Therapy for Neurological Disorders; Methods and Protocol Fredric P. Manfredsson Book 2016 Springer Science+Business Media New York 201

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發(fā)表于 2025-3-21 18:45:13 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Gene Therapy for Neurological Disorders
副標題Methods and Protocol
編輯Fredric P. Manfredsson
視頻videohttp://file.papertrans.cn/382/381981/381981.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Gene Therapy for Neurological Disorders; Methods and Protocol Fredric P. Manfredsson Book 2016 Springer Science+Business Media New York 201
描述.This volume provides a clear and detailed roadmap of how to design and execute a gene therapy experiment in order to obtain consistent results. Chapters in this book disseminate bits of unknown information that are important to consider during the course of experimentation and will answer questions such as: What delivery vehicle do you use?; How will you ensure that your vector retains stability?; What expression system best fits your needs?; What route will you choose to deliver your gene therapy agent?; How will you model the neurodegenerative disorder that you aim to investigate and what are the proven methods to treat these disorders in preclinical models? Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Authoritative and thorough, .Gene Therapy for Neurological Disorders: Methods and Protocols, .is a compilation of protocols and instructive chapters intended to give researchers, clinicians, and students of all levels, a foundation
出版日期Book 2016
關鍵詞Gene Therapy; Germline Manipulations; Neurodegenerative Disorders; Neurological Disorders; Preclinical; T
版次1
doihttps://doi.org/10.1007/978-1-4939-3271-9
isbn_softcover978-1-4939-4896-3
isbn_ebook978-1-4939-3271-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Small-Scale Recombinant Adeno-Associated Virus Purification purification from ten to twenty 15 cm plates of human embryonic kidney-derived 293B cells (HEK 293) cells that can yield approximately 300 μl of a 5?×?10. to 1?×?10. genome copies/ml viral preparation final concentration.
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https://doi.org/10.1007/978-1-4842-2793-0expression has its own strengths and limitations, which makes them more or less suitable for different applications. In this chapter, we describe the available alternatives and provide tips on how they can be implemented.
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S. Ferson,L. R. Ginzburg,R. A. Goldstein of virions for which each particular capsid variant is matched with the particular capsid gene encoding it; recovery of capsid gene sequences from target tissue after systemic administration. Prevalent variants are then analyzed and evaluated.
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