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Titlebook: Gene Targeting Protocols; Eric B. Kmiec Book 2000 Springer Science+Business Media New York 2000

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樓主: Buren
41#
發(fā)表于 2025-3-28 18:02:45 | 只看該作者
Danielle Goldfarb,Katherine M. Sharkeylkylate complex genomic DNA under physiological conditions. The alkylating event, which is detected using a quantitative version of the ligation-mediated polymerase chain reaction (LM-PCR) (.,.), acts as a marker for complex formation, and permits an estimation of the gene targeting frequency. Becau
42#
發(fā)表于 2025-3-28 20:58:32 | 只看該作者
https://doi.org/10.1007/978-3-030-20051-0in transgenic plants. These genes provide a means to select plant cells which have acquired the linked foreign gene that encodes a useful trait; selection genes are an unavoidable consequence of conventional transformation technologies. Reported herein are strategies to create, and select for, modif
43#
發(fā)表于 2025-3-28 22:56:42 | 只看該作者
Cinematic Perspectives on Digital Culture molecular medicine, that is, gene therapy and intracellular immunization. Among the major steps involved in the modification of target cells for these applications, gene transfer and the delivery of molecular medicine has become more and more critical.
44#
發(fā)表于 2025-3-29 04:19:32 | 只看該作者
45#
發(fā)表于 2025-3-29 08:46:24 | 只看該作者
Gene Transfer and Drug Delivery by Electronic Pulse Delivery, molecular medicine, that is, gene therapy and intracellular immunization. Among the major steps involved in the modification of target cells for these applications, gene transfer and the delivery of molecular medicine has become more and more critical.
46#
發(fā)表于 2025-3-29 12:11:01 | 只看該作者
47#
發(fā)表于 2025-3-29 17:56:57 | 只看該作者
Nucleic Acid Transfer Using Cationic Lipids,n recent years. The principal reasons for this have been the ease with which the methodology can be applied to a wide range of cell types; the relatively low cytotoxicity compared to other techniques; the high efficiency of nucleic acid transfer in comparison with methods such as calcium phosphate o
48#
發(fā)表于 2025-3-29 21:54:06 | 只看該作者
49#
發(fā)表于 2025-3-29 23:57:24 | 只看該作者
Gene Transfer and Drug Delivery by Electronic Pulse Delivery, molecular medicine, that is, gene therapy and intracellular immunization. Among the major steps involved in the modification of target cells for these applications, gene transfer and the delivery of molecular medicine has become more and more critical.
50#
發(fā)表于 2025-3-30 04:35:28 | 只看該作者
Strategies for Improving the Frequency and Assessment of Homologous Recombination,human clinical trials has been to produce long-term expression of therapeutic genes by integration of the DNA sequences encoding these genes into the chromosomes. Integration strategies include random, site-specific, and precise integration. To date, only random integration of retroviral vectors has
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