Titlebook: Gene Knockout Protocols; Wolfgang Wurst,Ralf Kühn Book 2009Latest edition Humana Press 2009 DNA.ES cells.Gene modification.Genetically eng

只看該作者 · 發(fā)表于 2025-3-25 14:50:36

Manipulating Mouse Embryonic Stem Cellsy after extensive in vitro manipulations. Here we provide straightforward protocols for proper care of these cells. Special emphasis is placed on aspects that are particularly critical for proper culture of this cell type.

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Cloning of ES Cells and Mice by Nuclear Transfered relatively easily from a variety of mouse genotypes and cell types. Such ntES cells can be used not only for experimental models of human therapeutic cloning but also as a means of preserving mouse genomes instead of preserving germ cells. Here, we describe our most recent protocols for mouse cloning.

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Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryosently developed applications of various cell types and origins used in the production of chimeras. Finally, detailed protocols and trouble-shooting suggestions will be presented for each of the steps involved.

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