| 書目名稱 | Gene Knockout Protocols | | 編輯 | Wolfgang Wurst,Ralf Kühn | | 視頻video | http://file.papertrans.cn/382/381936/381936.mp4 | | 概述 | Comprehensive guide to all techniques required for the generation of classical and conditional knockout mouse strains.Includes techniques for ES cell establishment, ES cell in vitro differentatio, and | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | Following the completion of the mouse and human genome sequences, a major challengeisthefunctionalcharacterizationofeverymammaliangeneandthedeciph- ing of their molecular interaction network. The mouse offers many advantages for the use of genetics to study human biology and disease, unmatched among other m- mals. Its development, body plan, physiology, behavior, and diseases have much in common, based on the fact that 99% of the human genes have a mouse ortholog. The investigation of gene function using mouse models is based on many years of tech- logical development. In the two decades since gene targeting in murine embryonic stem (ES) cells was first described by Mario Capecchi and colleagues, more than 3000 predesigned mouse mutants have been developed. To date, a variety of mouse mutagenesis techniques, either gene- or phenotype-driven, are used as systematic approaches. The availability of the genome sequence supports gene-driven approaches such as gene-trap and targeted mutagenesis in ES cells, allowing efficient and precise gene disruption. In combination with the use of site-specific DNA recombinases, in particular the Cre/loxP system, gene disruptioncan be directed to spe | | 出版日期 | Book 2009Latest edition | | 關(guān)鍵詞 | DNA; ES cells; Gene modification; Genetically engineered mice; Mouse genome; Mutagenesis techniques; Mutan | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-59745-471-1 | | isbn_softcover | 978-1-62703-834-8 | | isbn_ebook | 978-1-59745-471-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2009 |
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