Titlebook: Gene Knockout Protocols; Martin J. Tymms,Ismail Kola Book 2001 Humana Press 2001

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Manipulating Mouse Embryonic Stem Cells,enome and to generate mice with specific targeted gene mutations. The improvement in the quality of reagents (embryonic stem (ES) cells and media) available to the scientific community has made this technology accessible to many laboratories.

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Gene Targeting in a Centralized Facility,re relatively straightforward and many of the experimental procedures are standardised (.–.). A further step toward increasing the efficiency in the generation of knockout mice lies in the pooling of resources and knowledge by establishing a centralized gene-targeting facility.

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Overview,f gene products. Not only can gene disruption demonstrate the function of genes, but by disrupting cell development or survival it can also demonstrate the function of specific cell types. Some knockout mice are murine models of human genetic diseases and have proven that a single gene defect is cap

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Isolation and Maintenance of Primate Embryonic Stem Cells,er particular conditions, these cells differentiate into derivatives of endoderm, mesoderm, ectoderm, and trophoblast (.,.). In mammals, many developmental events are studied using mouse embryos or ES cells, but some aspects of development differ significantly between humans and mice, such as the ti