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Titlebook: Gene Expression Profiling; Richard A. Shimkets Book 2004 Humana Press 2004

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發(fā)表于 2025-3-21 19:47:51 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)Gene Expression Profiling
編輯Richard A. Shimkets
視頻videohttp://file.papertrans.cn/382/381919/381919.mp4
概述Includes supplementary material:
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Gene Expression Profiling;  Richard A. Shimkets Book 2004 Humana Press 2004
描述Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip?, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay?, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.
出版日期Book 2004
版次1
doihttps://doi.org/10.1385/1592597513
isbn_softcover978-1-61737-429-6
isbn_ebook978-1-59259-751-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2004
The information of publication is updating

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a variety of measurements that different technologies may provide. Indeed, there are many reasons that applying different technologies to transcript abundance may give different results. This may result from an incomplete understanding of the gene in question or from shortcomings in the applications
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發(fā)表于 2025-3-22 02:13:36 | 只看該作者
China Ethnic Statistical Yearbook 2016various measurements that a variety of different technologies provide. Indeed, there are many reasons why applying different technologies to the problem of transcript abundance may give different results, owing to an incomplete understanding of the gene in question or from shortcomings in the applic
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Entertainment and Other Cultural Activity,liland et al. StaRT-PCR allows rapid, reproducible, standardized, quantitative measurement of data for many genes simultaneously. An internal standard CT is prepared for each gene, cloned to generate enough for >10. assays and CTs for up to 1000 genes are mixed together. Each target gene is normaliz
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https://doi.org/10.1007/978-1-137-29393-0ce information to determine the potential function of novel genes captured. The method relies on transcript visualization coupled to a database query to rapidly and quantitatively identify differentially expressed transcripts. The method has been applied to a wide variety of disease models in a vari
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發(fā)表于 2025-3-23 01:46:08 | 只看該作者
Chinese Academy of Cyberspace Studies of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effect and combines normalization and subtraction in a si
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發(fā)表于 2025-3-23 08:13:00 | 只看該作者
Chinese Academy of Cyberspace Studiesictor Velculescu et al.., SAGE has been widely used. Recently, the efficiency of the method has been emphasized as a means to identify novel transcripts or genes that are difficult to identify by conventional methods. SAGE is based on the principle that a 10-base pair (bp) cDNA fragment contains suf
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