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Titlebook: Gene Correction; Methods and Protocol Francesca Storici Book 2014 Springer Science+Business Media, LLC 2014 gene correction.gene repair.gen

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發(fā)表于 2025-3-21 17:35:39 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Gene Correction
副標題Methods and Protocol
編輯Francesca Storici
視頻videohttp://file.papertrans.cn/382/381906/381906.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Gene Correction; Methods and Protocol Francesca Storici Book 2014 Springer Science+Business Media, LLC 2014 gene correction.gene repair.gen
描述.Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. .Gene Correction: Methods and Protocols .provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Gene Correction: Methods and Protocols .seeks to serve scientists of all backgrounds interested in the area
出版日期Book 2014
關(guān)鍵詞gene correction; gene repair; gene targeting; human genome; human pluripotent stem cells
版次1
doihttps://doi.org/10.1007/978-1-62703-761-7
isbn_softcover978-1-4939-6321-8
isbn_ebook978-1-62703-761-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2014
The information of publication is updating

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Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophilaspecific integrase mediated repeated targeting (SIRT) method, which combines homologous recombination, site-specific integration, and bacterial recombineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epit
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Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombinationof human development, disease pathogenesis, and the generation of screening systems to identify novel therapeutic agents. Autologous cell therapies based on patient-derived induced pluripotent stem cells also hold great promise for the treatment and correction of many inherited and acquired diseases
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Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cellsication for the treatment of many disorders. Among those, Duchenne muscular dystrophy (DMD) represents an ideal candidate for gene editing primarily due to the large size of dystrophin, the gene responsible for the disease, which limits the use of gene replacement approaches. Critical in the evaluat
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Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotiing a specific target sequence in vivo, driving that site into an exploitable repair pathway. Information is transferred to the site via participation in the pathway by the second component, a donor nucleic acid, resulting in a permanent change in the target sequence. We have developed biologically
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Triplex-Mediated Genome Targeting and Editingnous recombination machinery. The former includes a repertoire of sequence-specific binding molecules for targeted induction and appropriation of this machinery, such as by triplex-forming oligonucleotides (TFOs) or triplex-forming peptide nucleic acids (PNAs) and recombinagenic donor DNA, respectiv
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Targeting , Transposon Integrations in the Human Genometing such systems to achieve site-directed integration as compared to un-targeted integration which occurs with native or unmodified transposon systems. The naturally active . transposon system is derived from insects but has been shown to be very efficient in gene-modifying human cells. Recent effo
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