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Titlebook: Gap Junction Protocols; Mathieu Vinken,Scott R. Johnstone Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 Con

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發(fā)表于 2025-3-21 18:55:26 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)Gap Junction Protocols
編輯Mathieu Vinken,Scott R. Johnstone
視頻videohttp://file.papertrans.cn/381/380704/380704.mp4
概述Includes cutting-edge methods and protocols involving gap junctions.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Gap Junction Protocols; Mathieu Vinken,Scott R. Johnstone Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 Con
描述Presentingstate-of-the-art protocols to study gap junctions, this detailed book firstfocuses on the use of methods and tools to investigate the different aspects ofconnexin expression and gap junction regulation. The second part of the volumedescribes several methods to probe gap junction functionality as such. Writtenin the highly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step and readily reproducible laboratoryprotocols, and key tips on troubleshooting and avoiding known pitfalls.?.Authoritativeand practical, Gap Junction Protocols is intended for basic and appliedresearchers in the area of biomedical and life sciences, both in academic andindustrial settings..
出版日期Book 2016
關(guān)鍵詞Connexin expression; Cell signaling; Connexons; Gap junctional intercellular communication; GJIC regulat
版次1
doihttps://doi.org/10.1007/978-1-4939-3664-9
isbn_softcover978-1-4939-8110-6
isbn_ebook978-1-4939-3664-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2016
The information of publication is updating

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Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Im by carrying out multiple photo-activations in a coupled cell pair, LAMP assay can track changes in cell coupling strength between coupled cells, hence providing a powerful method for investigating the regulation of junctional coupling by cellular biochemical changes.
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Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junave propagation was assayed by imaging fluorescent calcium in bovine corneal endothelial cells loaded with a fluorescent calcium dye using a confocal microscope. Spatial changes in intercellular calcium concentration following mechanical stimulation were measured in the mechanical stimulated cell an
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Florent de Dinechin,Martin Kumm by carrying out multiple photo-activations in a coupled cell pair, LAMP assay can track changes in cell coupling strength between coupled cells, hence providing a powerful method for investigating the regulation of junctional coupling by cellular biochemical changes.
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DNA Methylation Analysis of Human Tissue-Specific Connexin Genes,hylation of connexin genes, play important roles in regulating gene expression. Over the past decade, several methods have been applied to characterize DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in huma
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Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoressearch field. The present chapter describes the setup of an immunoblot procedure, including protein extraction and quantification from biological samples, gel electrophoresis, protein transfer, and immunoblotting, which is optimized for analysis of connexins in liver tissue. In essence, proteins are
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