Titlebook: Gangliosides; Methods and Protocol Sandro Sonnino,Alessandro Prinetti Book 2018 Springer Science+Business Media, LLC, part of Springer Natu

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Synthesis of Fluorescent Gangliosides,, and their interaction with membrane lipids and proteins. Here I describe established procedures to synthesize ganglioside derivatives with a fluorescent tag either attached to its sialooligosaccharide or ceramide portion. These procedures are chosen as to minimize the integrity of the ganglioside

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Synthesis of Photoactivatable and Paramagnetic Gangliosides, photoactivatable and paramagnetic ganglioside derivatives have proved to be invaluable tools. Here, protocols for the synthesis of such ganglioside derivatives are described. These derivatives bear in their ceramide portion either a highly photoreactive (3-trifluoromethyl)phenyldiazirinyl- or a spi

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Methods for Assay of Ganglioside Catabolic Enzymes,dence suggests an important role of the sphingolipid catabolic enzymes associated with both lysosomes and plasma membrane. Over the years, several cell-free and cell-based assays are developed in order to evaluate the activities both intracellularly and at the cell surface. Here, we propose a select

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978-1-4939-9323-9Springer Science+Business Media, LLC, part of Springer Nature 2018

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Chemical and Physicochemical Properties of Gangliosides,In this chapter, we briefly describe the structural features of gangliosides, and focus on the peculiar chemicophysical features of gangliosides, an important class of membrane amphipathic lipids that represent an important driving force determining the organization and properties of cellular membranes.