Titlebook: G Protein-Coupled Receptor Signaling; Methods and Protocol Mario Tiberi Book 2019 Springer Science+Business Media, LLC, part of Springer Na

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Mario TiberiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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G Protein-Coupled Receptor Signaling978-1-4939-9121-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-8349-4634-8olution of G protein-coupled receptors (GPCRs) has made several of these difficult to express membrane proteins amenable to prokaryotic expression. Here, we describe a protocol for near complete .CH.-methionine labeling of a thermostable neurotensin receptor 1 (enNTS.) variant in . for solution NMR-

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Selbstreflexion und (Selbst-)Wahrnehmung,his context, we present two methods for the site-specific fluorescent labeling of difficult-to-express membrane proteins in combination with cell-free protein synthesis. The cell-free protein synthesis system is based on Chinese Hamster Ovary Cells (CHO) since this system contains endogenous membran

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