| 書(shū)目名稱(chēng) | Fluorescent Energy Transfer Nucleic Acid Probes | | 副標(biāo)題 | Designs and Protocol | | 編輯 | Vladimir V. Didenko | | 視頻video | http://file.papertrans.cn/345/344725/344725.mp4 | | 概述 | Includes supplementary material: | | 叢書(shū)名稱(chēng) | Methods in Molecular Biology | | 圖書(shū)封面 |  | | 描述 | Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called F?rster tra- fer, or by non-F?rster transfer mechanisms. Although the probes using F?rster transfer were developed and | | 出版日期 | Book 2006 | | 關(guān)鍵詞 | DNA; Genotyp; Mutation; Single Nucleotide Polymorphism; Termination; gene expression; genes; hybridization | | 版次 | 1 | | doi | https://doi.org/10.1385/1597450693 | | isbn_softcover | 978-1-61737-531-6 | | isbn_ebook | 978-1-59745-069-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2006 |
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